Los factores genéticos determinantes de la resistencia a la infección por VIH y del control de la progresión al SIDA: Implicaciones sobre la patogénesis y las estrategias terapéuticas para la erradicación del VIH. Una revisión

Esta revisión describe y discute los factores genéticos que al menos en parte, determinan la resistencia a la infección y que controlan el curso progresivo de la enfermedad en las personas infectadas por el virus de la inmunodeficiencia humana. Los factores genéticos podrían explicar la no progresión o la progresión lenta de la enfermedad, de una proporción de individuos infectados por VIH denominados no progresores a largo plazo. En general, este grupo permanece sin síntomas durante más de 10 años, mantiene estable sus niveles circulantes de cT CD4+ y habitualmente tiene baja carga viral. No obstante que los fenómenos de la no progresión y de la progresión rápida son aún incomprendidos, es probable que ciertos alelos de clase I y clase II del complejo principal de histocompatibilidad se asocien con un riesgo menor o mayor para desarrollar el síndrome de inmunodeficiencia adquirida...

System of 14C-glucose incorporation in axenic cultures of Entamoeba invadens: IP-1 strain

Background. It has been described that the walls of the amebic cyst from Entamoeba invadens are composed mainly of chitin, a polysaccharide of amino-sugars. It is also know that the synthesis of this polysaccharide is closely related to the degradation of the intracellular glycogen in this organisms. Nevertheless, it is not know whether the intracellular glycogen is really the source of the glucose requirements for the synthesis of the cell wall. Methods. To determine the relationship between the wall cyst synthesis and glycogen degradation, it was considered to develop an in vitro culture cell system to label this polysaccharide with radioactive glucose. In this study, a system of 14C-glucose incorporation in axenic cultures of E. invaden was developed. The experiments in the study were carried out to recognize if an increase occurred in the 14C-glucose incorportation into ameba when the amount of the radioctivity used was increased, or whether this incorporation is a dependent metabolic stage. Results. The results showed that the amount of glucose incorportation reached similar values of 4.5 x 10-12 mmol per cell in both cases. A differente slope in the glucose kinetic incorporation between the cultures previously subjected to glucose depletion and the standard cultures was observed. Conclusions. This axenic method of radioactive glucose incorporation in Entamoeba invadens could facilitate the analysis on a greater scale of the metabolism of this nutrient